Table 2. Plasmids Used and Created in This Study (All Plasmid Files are Available as .gbk in the Supporting Information).

name	relevant features	parental plasmid	reference
pSIM5	λ red expression vector, pSC101 ori, repAts, TetR		(64)
pBAD	modified pBAD-TOPO, AmpR		(65)
pSL0001	RybB seed region acceptor vector for PBAD expression, AmpR	pBAD	this work
pSL0002	seed and scaffold-acceptor vector for PBAD expression, AmpR	pSL0001	this work
pSL0003	empty control vector with PBAD, KanR	pSL0001	this work
pSL0004	RybB seed region acceptor vector for PBAD expression, KanR	pSL0002	this work
pSL0005	seed and scaffold-acceptor vector for PBAD expression, KanR	pSL0002	this work
pSL0006	empty control vector, AmpR	pSL0001	this work
pSL0007	promoter and seed region acceptor vector, AmpR	pSL0006	this work
pSL0008	promoter, seed, and scaffold-acceptor vector, AmpR	pSL0006	this work
pSL0009	empty control vector, KanR	pSL0003	this work
pSL0010	promoter and seed region acceptor vector, KanR	pSL0009	this work
pSL0011	promoter, seed, and scaffold-acceptor vector, KanR	pSL0009	this work
pSL0137	derivative of pSL0011, SapI recognition sites instead of BbsI, KanR	pSL0011	this work
pSLcol_01	pSL0004 with various seeds (Table S1), KanR	pSL0004	this work
p-PL	PLlacO-1, KanR	pSL0137	this work
p-PL-RybB-s8	RybB-s8 expression from PLlacO-1, KanR	pSL0010	this work
p-PL-RybB-s38	RybB-s38 expression from PLlacO-1, KanR	pSL0010	this work
p-PL-RybB-wt	wild-type RybB expression from PLlacO-1, KanR	pSL0010	this work
p-PL-RybB-Δseed	RybB scaffold expression from PLlacO-1, KanR	pSL0010	this work
p-PL-MicA-s8	MicA-s8 expression from PLlacO-1, KanR	pSL0011	this work
p-PL-MicA-Δseed	MicA scaffold expression from PLlacO-1, KanR	pSL0011	this work
p-PL-MicF-s8	MicF-s8 expression from PLlacO-1, KanR	pSL0011	this work
p-PL-MicF-Δseed	MicF scaffold expression from PLlacO-1, KanR	pSL0011	this work
p-PL-OmrB-s8	OmrB-s8 expression from PLlacO-1, KanR	pSL0011	this work
p-PL-OmrB-Δseed	OmrB scaffold expression from PLlacO-1, KanR	pSL0011	this work
p-PL-RybB-s28	RybB-s28 expression from PLlacO-1, KanR	pSL0010	this work
p-PL-RybB-s8-PL-RybB-s28	RybB-s8 and RybB-s28 expression from PLlacO-1, KanR	pSL0011	this work
p-PL-RybB-s8-PJ-RybB-s28	RybB-s8 and RybB-s28 expression from PLlacO-1 and PBBa_J23119, respectively, KanR	pSL0011	this work
p-PL-RybB-s8-PL-MicA-s28	RybB-s8 and MicA-s28 expression from PLlacO-1, KanR	pSL0011	this work
p-PL-RybB-s8- PJ-MicA-s28	RybB-s8 and MicA-s28 expression from PLlacO-1 and PBBa_J23119, respectively, KanR	pSL0011	this work