Table 1.
Multiplexed panels
| Antibody | Clone | Source | Dilution | Opal Fluorophore | |
|---|---|---|---|---|---|
| 9-color panel | CD68 | KP1 | Dako | 1:100 | Opal-540 |
| CD8 | C8/144B | Dako | 1:2500 | Opal-620 | |
| FoxP3 | D2W8E | Cell Signalling | 1:100 | Opal-570 | |
| CD163 | 10D6 | Leica Biosystems | 1:150 | Opal-690 | |
| CD103 | EPR4166(2) | Abcam | 1:500 | Opal-480 | |
| PD-1 | EPR4877–2 | Abcam | 1:100 | Opal-520 | |
| PD-L1 | E1L3N | Cell Signalling | 1:200 | Opal-650 | |
| Pan-cytokeratin (CK) | AE1/AE3 | Dako | 1:300 | Opal-780 | |
| 6-color panel | CTLA-4 | UMAB249 | Biomedical care | 1:100 | Opal-520 |
| CD4 | 4B12 | ThermoFisher | 1:20 | Opal-650 | |
| CD8 | C8/144B | Dako | 1:2500 | Opal-480 | |
| Pan-HLA class I (HLA-I) | EMR8–5 | Abcam | 1:100 | Opal-570 | |
| Pan-cytokeratin (CK) | AE1/AE3 | Dako | 1:300 | Opal-690 |