FIG. 1.

(A) Schematic representation of Tn2000 that contained In53 from E. coli MG-1. The 5′ and the 3′-CSs are indicated by a double arrow. ORFs or genes are shown as boxes with an arrow indicating the orientation of the coding sequence and with the gene name above the corresponding box. The different promoter sequences, P₂, P₃, P₄, P₅, P_qacI, P_cmlA, and P_oxa10 are represented with a gray box and with an arrow. The hatched boxes at each end of the transposon represent the target site duplication. IS26-related inverted right and left repeats are shown by empty and filled triangles, respectively. The coding orientation of the IS26 transposase gene is represented by an arrow. The Tn2000 and the In53 structures are indicated between two divergent arrows. (B) Schematic representation of pRLT-2 and pRLT-3, two plasmids carrying large DNA inserts from E. coli MG-1 cloned into pBKCMV. The thick dotted line represents the pBKCMV vector sequence. (C) Schematic representation of pRLT-4 to -10, which correspond to plasmids constructed from various PCR products cloned into pPCRscript vectors (either Amp^r or Cm^r). Each construct exists in both orientations (a and b) with respect to P_lac, the plasmid-located promoter.