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. 2022 Sep 2;22:232. doi: 10.1186/s12906-022-03707-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — ERK and AKT signaling are key regulators in WEAOR-mediated antiphotoaging activity. Pretreatment of WEAOR to NIH-3T3 cells attenuated phosphorylation of ERK and AKT, but not that of JNK and p38 MAPK. Representative figures of Western blotting are shown (A), and the intensity of each band was estimated using ImageJ. The relative expression rates of ERK (p-ERK/t-ERK), p38 (p-p38/p38), JNK (p-JNK/JNK), and AKT (p-AKT/AKT) were calculated and tabulated (B). Experiments were conducted in triplicate and repeated three times with similar results. Each data value is expressed as mean ± SD. Different letters indicate significant differences between groups (P < 0.05)