FIG. 3.

Phosphorimage of a thin-layer chromatogram showing [¹⁴C]nicotinamide release from [carbonyl-¹⁴C]NAD. Reactions of different dinitrogenase reductases with DRAT were performed as described in Materials and Methods except that different proteins were added to the reaction as follows: lane 1, DRAT only; lane 2, DRAT plus A. vinelandii dinitrogenase reductase; lane 3, DRAT plus wild-type R. rubrum dinitrogenase reductase; lane 4, DRAT plus R101F dinitrogenase reductase.