TABLE 2.
Strain | Plasmidsd | Luciferase activity (relative light units) ± SDa with:
|
Induction factorc | |
---|---|---|---|---|
No inducer | 2-HBPb | |||
ET8000 | pHYBP103, pHYBP124 | 7.48E+04 ± 1.27E+04 | 1.49E+06 ± 2.21E+05 | 20 |
pHYBP106, pHYBP124 | 1.89E+05 ± 9.49E+03 | 1.34E+06 ± 1.26E+05 | 7.1 | |
ET8045e | pHYBP103, pHYBP124 | 8.50E+04 ± 5.00E+03 | 8.11E+04 ± 7.94E+03 | 0.95 |
pHYBP106, pHYBP124 | 2.39E+05 ± 1.57E+04 | 2.49E+05 ± 1.02E+04 | 1.1 |
All values were obtained in two independent experiments, each performed in triplicate.
Assay concentration for 2-HBP was 0.2 mM.
Induction factor is the quotient of the bioluminescence measured with and without the inducer.
pHYBP103 contains hbpC::luxAB; pHYBP106 contains hbpD::luxAB; pHYBP124 contains hbpR.
RpoN mutant.