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Comparison of PagR1 and PagR2 Kds. Purified recombinant PagR1 and PagR2 were incubated at increasing concentrations with 0.2 nM biotin-labeled probes. The probe binding of (A) Psap and (B) Peag proximal were calculated as a fraction of bound promoter DNA over the total promoter DNA. Increasing binding with increasing protein concentration was plotted, and Kds were calculated using the Hill equation. Kds are reported with 95% confidence intervals.
