FIG 4.

Moxifloxacin-mediated respiration arrest reversed by NAC. (A) OCR (pmol/min). Exponentially growing M. tuberculosis cultures were either left untreated (UT) or treated with 10× MIC of moxifloxacin (MOXI; 5 μM) for the indicated times; black dotted lines indicate when MOXI, NAC (1 mM), or CCCP (10 μM) was added. Determination was done with a Seahorse XFp analyzer. Data are percentages of third baseline values. (B) ECAR (mpH/min) (H⁺ production or extracellular acidification due to glycolytic and TCA flux). Determination was as for OCR, with data representing percentages of third baseline values. (C and D) NAC (1 mM) addition enhanced OCR of (C) untreated and (D) MOXI-treated cells. Data are representative of two independent experiments performed in triplicate.