Figure 9.

The effect of delphinidin on the elevated mRNA transcript levels of copper transporters Ctr1 and ATP7A in MCF-10A-Cu cells compared to their parental MCF-10A cells. TRIzol (Invitrogen) was used to isolate total RNA in accordance with the manufacturer’s instructions. As described in “Methods,” Ctr1 and ATP7A mRNA expression was quantified using real-time PCR. To determine the effect of delphinidin on mRNA expression, only MCF-10A-Cu cells (normal MCF-10A cells cultured in a medium containing 25 µM CuCl2) with enhanced mRNA expression of copper transporters were treated with 50 µM delphinidin. The values reported are the S.E. of three separate trials. p ≤ 0.01 when compared to respective untreated control was treated as significant.