Figure 3.

ATAC-seq analysis of cardiac fibroblasts of different differentiation states.

Tcf21 lineage-traced cardiac fibroblasts were sorted from the uninjured myocardium and the infarct at different time points after MI for ATAC-seq analysis. (a) Enrichment of ATAC-seq peaks around TSS and TES of genes. (b) Relative abundance of ATAC-seq peaks in different genomic features. (c-d) PCA analysis (c) and Poisson distance analysis (d) of ATAC-seq data show tight clustering of repeats of each group. (e-f) Venn diagram showing the overlaps between ATAC-seq peaks that had different abundances between Tcf21 lineage–traced fibroblasts isolated from the uninjured myocardium and those from the infarct at different time points after MI. Peaks that were upregulated after MI compared to cardiac fibroblasts isolated from the uninjured are shown in E. Downregulated peaks are shown in F. Uninj, uninjured; D3, day 3; D7, day 7; W2, week 2; W4, week 4.