Skip to main content
. 2022 Sep 6;9:897695. doi: 10.3389/fmed.2022.897695

Figure 2.

Figure 2

Effect of cell culture systems on the expression of SARS-CoV-2 entry genes in primary human airway epithelial cells. Human bronchial epithelial cells (HBEC) (A) and small airway epithelial cells (SAEC) (B) derived from the same donors were submerged cultured (SM) or differentiated under air-liquid interface (ALI) conditions (4 patients, 6 cultures). Gene expression of SARS-CoV-2 entry genes including ACE2, TMPRSS2, CTSL and AXL was determined by LightCycler PCR and normalized to HRPT expression. Expression of the four SARS-CoV-2 genes in HBEC and SAEC cultured in submerged culture was compared with those in ALI culture. Statistical significance was determined by paired t test (n = 6). *p < 0.05 and **p < 0.01.