TABLE 2.
Categorized functions of identified proteins showing different expression levels under PHB-producing versus non-PHB-producing conditions
Spot no. | Category | Gene | Proteina | pl/mol massb |
---|---|---|---|---|
Cell process | ||||
1 | Chaperoning | dnaK | DnaK, chaperone for Hsp70 | 4.81/69.6 |
2 | Chaperoning | groEL | GroEL, chaperone for Hsp60 | 4.85/57.3 |
11 | Chaperoning | groES | GroES, chaperone for Hsp10 | 5.15/10.4 |
6 | Transport-binding | rbsB | RbsB, d-ribose periplasmic binding protein | 6.85/30.9 |
Energy metabolism | ||||
4 | Glycolysis | fba | Fba, fructose-bisphosphate aldolase, class II | 5.52/39.2 |
7 | Glycolysis | gpmA | GpmA, phosphoglycerate mutase | 5.85/28.5 |
8 | Glycolysis | tpiA | TpiA, triosephosphate isomerase | 5.64/27.0 |
9 | Entner-Doudoroff pathway | eda | Eda, 2-oxo-3-deoxy-6-phosphogluconate aldolase (2-keto-4-hydroxyglutarate aldolase) | 5.57/22.3 |
Central intermediary metabolism | ||||
5 | General | kba | Kba, d-tagatose-1,6-bisphosphate aldolase | 5.98/31.1 |
Macromolecular metabolism | ||||
3 | Protein translation and modification | tufA | EF-Tu, protein chain elongation factor | 5.3/43.3 |
Other | ||||
10 | Adaptation | dps | Dps, DNA protection protein during starvation | 5.72/18.7 |
13 | Broad regulatory functions | relB | RelB, negative regulator of translation | 4.81/9.1 |
12 | Unknown function | yfiD | YfiD, 14.3-kDa hypothetical protein | 5.09/14.3 |
Proteins were identified by the peptide fingerprinting method. The ProteinProspector server was used for the identification of proteins, and SWISS-PROT was used as a reference database.
Molecular masses are shown in kilodaltons.