Fig. 5.

(a) Cell viability after incubation with bisphenols for a grass carp hepatocyte cell line (L8824) and breast adenocarcinoma cells (MCF-7). ∗∗: p < 0.01. The IC₅₀ values of BPA, BPAF, BPB, BPF and BPS were 16.19 ± 3.22, 3.43 ± 0.54, 9.67 ± 0.92, 11.29 ± 2.12 and 35.89 ± 6.01 μM, respectively, for L8824, while the corresponding values of 36.88 ± 4.46, 7.87 ± 3.98, 21.65 ± 4.92, 31.89 ± 1.69 and 57.92 ± 7.76 μM, respectively, were obtained for MCF-7. (b) Bioaccessibility of the bisphenols from the bisphenol-attached PVC microplastics in the water (striped bars) and warm-I conditions (gray bars). The bioaccessibility was calculated as the percentage of desorbed bisphenols to the total adsorbed bisphenols on the PVC microplastics (with initial bisphenols concentration as 2.0 mg·L⁻¹).