Histogram of difference between the alpha diversity indices of groups in soil surrounding microplastics and on microplastic surfaces at different soil layers. (a)bacteria, (b)fungi. S-A and MP-A represent soil and microplastic samples, respectively, in the humus horizon; S-B and MP-B represent soil and microplastic samples, respectively, in the eluvial horizon. ∗∗∗p < 0.001, ∗∗p < 0.01, ∗p < 0.05.