Figure 4.

TLR2 mediates the activation of MAPK signaling pathways in S. uberis infection. The neutrophils were pretreated with TLR2-antibody (anti-TLR2, 100 ng/mL) for 1 h before infected with S. uberis at MOI of 10. (A) Immunoblot and statistical analysis of neutrophil p38, ERK, JNK and p47^phox proteins and phosphorylated proteins at 2 h. (B) The Intracellular ROS level evaluated by Flow Cytometry at 2 h. (C) The dsDNA content in supernatant at 4 h. Data are presented as mean ± SEM (n = 3). *P < 0.05.