FIGURE 4.

Effect of modulation of the S1P lyase (SPL) expression level on peritoneal cancer dissemination. Signal (A, D and G) and area (B, E and H) of the disseminated peritoneal cancer were analysed using the Odyssey CLx Imaging system. Identical illumination settings (channels: 700/800, resolution: 42 μM, intensities: auto, quality: lowest, analysis: small animal, and focus: 2.0 mm) were used for acquiring all images. (C, F and I) Representative scanned images from each group. Images were acquired and analysed using Image Studio Ver. 4.0 software. Differences between the two groups were statistically analysed by the unpaired Student's t‐test. EV, empty vector transfected control cell line; HR, homologous recombination vector only transfected control cell line; SPL, SPL overexpressing cell line; SPL HR, homologous recombination vector transfected SPL overexpressing cell line; Cr/Cas SPL, SPL knock out cell lines by using CRIPR/Cas9 system; SPL Cr/Cas GPR55, GPR55 knock out cell lines by using CRIPR/Cas9 system in SPL overexpressing cell line