FIGURE 4.

Quantification of protein phosphorylation. (A) Sketch of the assessed phosphorylation of signaling proteins being involved in GLUT-4 mediated glucose import as induced by contraction and insulin, angiotensin and cytokine signaling. Abbreviation: AT1, angiotensin receptor 1; CHO, glucose. (B–E) Images depicting the detection of the phosphorylation content for vastus lateralis muscle samples from an ACE-DD (B,C) and ACE-II (D,E) genotype ½ hrs post exercise in pairs of filters of the phospho-kinase arrays. Each phosphorylation content is assessed as measurement from a pair of spots. The position of the spot pairs for AKT-pS473 and AKT-pT308 is indicated. Strongest signals correspond to the reference signals used to ‘standardize’ the assessed counts.