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. 2022 Sep 6;13:933792. doi: 10.3389/fphys.2022.933792

TABLE 4.

Exercise-induced alterations of the muscle kinome in dependence of the ACE-I/D genotype.

phospho-protein time [min] ACE-DD ACE-ID/II ID/DD vs II ALL
Mean ± SD Mean ± SD p-value (30 or 480) p-value (30&480) Mean ± SD
AKT-pS473 30 6.72 ± 0.31 2.41 ± 1.01 <0.001 <0.001 3.27 ± 2.12
480 6.29 ± 0.29 4.14 ± 1.99 0.002 4.86 ± 1.87
 p-value (480 vs 30) 0.581 <0.001 0.153
AKT-pT308 30 0.61 ± 0.23 1.29 ± 0.57 0.099 0.048 1.16 ± 0.58
480 0.35 ± 0.13 1.12 ± 0.61 0.235 0.66 ± 0.67
 p-value (480 vs 30) 0.158 0.311 0.418
AMPKα1-pT183 30 1.24 ± 0.08 1.60 ± 0.16 0.563 0.503 1.52 ± 0.21
480 1.36 ± 0.09 1.62 ± 0.75 0.704 1.53 ± 0.55
 p-value (480 vs 30) 0.876 0.965 0.876
AMPKα2-pT172 30 3.58 ± 0.77 3.78 ± 0.48 0.742 0.409 3.74 ± 0.43
480 4.85 ± 1.04 3.90 ± 1.18 0.157 4.21 ± 1.00
 p-value (480 vs 30) 0.102 0.805 0.128 0.43
CHK2-pT68 30 2.47 ± 0.14 3.02 ± 0.16 0.375 0.557 2.91 ± 0.28
480 2.67 ± 0.15 2.38 ± 0.40 0.667 2.48 ± 0.33
 p-value (480 vs 30) 0.797 0.183 0.633
c-JUN-pS63 30 0.80 ± 0.32 1.03 ± 0.28 0.704 0.557 0.99 ± 0.26
480 0.44 ± 0.18 0.75 ± 0.48 0.653 0.65 ± 0.38
 p-value (480 vs 30) 0.647 0.548 0.482
CREB-pS133 30 1.68 ± 0.23 2.01 ± 0.14 0.589 0.697 1.94 ± 0.19
480 2.04 ± 0.28 2.06 ± 0.71 0.973 2.06 ± 0.51
 p-value (480 vs 30) 0.639 0.907 0.645
EGFR-pY1086 30 3.56 ± 0.42 4.55 ± 0.39 0.110 0.028 4.35 ± 0.55
480 3.02 ± 0.35 4.05 ± 0.68 0.128 3.70 ± 0.76
 p-value (480 vs 30) 0.486 0.293 0.253
eNOS-pS1177 30 0.41 ± 0.16 0.52 ± 0.20 0.868 0.786 0.50 ± 0.18
480 0.24 ± 0.09 0.38 ± 0.21 0.829 0.33 ± 0.17
 p-value (480 vs 30) 0.820 0.779 0.733
ERK1/2-pT202/Y204 30 2.35 ± 0.26 2.91 ± 0.32 0.366 0.364 2.80 ± 0.37
&T185/Y187 480 2.01 ± 0.22 2.28 ± 0.49 0.685 2.19 ± 0.38
 p-value (480 vs 30) 0.659 0.190 0.288
FAK-pY397 30 1.10 ± 0.04 1.57 ± 0.21 0.449 0.503 1.48 ± 0.28
480 1.16 ± 0.04 1.31 ± 0.25 0.830 1.26 ± 0.20
 p-value (480 vs 30) 0.939 0.584 0.826
FGR-pY412 30 0.76 ± 0.09 1.61 ± 0.52 0.167 0.172 1.44 ± 0.59
480 0.64 ± 0.08 1.04 ± 0.34 0.556 0.91 ± 0.33
 p-value (480 vs 30) 0.881 0.231 0.451
FYN-pY420 30 1.97 ± 0.14 3.01 ± 0.53 0.094 0.159 2.80 ± 0.65
480 2.19 ± 0.16 2.44 ± 0.48 0.705 2.36 ± 0.37
 p-value (480 vs 30) 0.781 0.239 0.704
GSK3α/β-pS21/S9 30 2.39 ± 0.22 3.15 ± 0.67 0.216 0.073 3.00 ± 0.67
480 2.10 ± 0.19 2.98 ± 1.05 0.192 2.69 ± 0.90
 p-value (480 vs 30) 0.711 0.721 0.616
HCK- pY411 30 3.56 ± 0.62 6.09 ± 1.11 <0.001 0.001 5.58 ± 1.49
480 4.56 ± 0.80 5.11 ± 0.99 0.411 4.93 ± 0.77
 p-value (480 vs 30) 0.199 0.043 0.973
HSP27-pS78/S82 30 3.23 ± 1.07 3.00 ± 0.41 0.710 0.973 3.05 ± 0.37
480 2.01 ± 0.66 2.27 ± 0.59 0.701 2.18 ± 0.44
 p-value (480 vs 30) 0.118 0.124 0.033
HSP60 30 0.49 ± 0.29 0.33 ± 0.20 0.797 0.284 0.36 ± 0.18
480 1.19 ± 0.70 0.37 ± 0.05 0.224 0.64 ± 0.48
 p-value (480 vs 30) 0.370 0.939 0.421
JNK-1/2/3 30 1.61 ± 0.05 2.14 ± 0.40 0.390 0.275 2.04 ± 0.42
-pT183/Y185, T221/Y223 480 1.54 ± 0.05 2.01 ± 0.64 0.488 1.85 ± 0.53
 p-value (480 vs 30) 0.923 0.775 0.817
LCK-pY394 30 1.27 ± 0.01 1.68 ± 0.27 0.501 0.661 1.60 ± 0.30
480 1.25 ± 0.01 1.23 ± 0.45 0.983 1.24 ± 0.32
 p-value (480 vs 30) 0.982 0.349 0.611
LYN-pY397 30 1.99 ± 0.35 2.80 ± 0.20 0.185 0.423 2.64 ± 0.40
480 2.55 ± 0.45 2.47 ± 0.31 0.899 2.50 ± 0.23
 p-value (480 vs 30) 0.467 0.481 0.800
MSK1/2-pS376/S360 30 3.64 ± 0.37 3.79 ± 0.24 0.807 0.457 3.76 ± 0.21
480 3.15 ± 0.32 3.68 ± 1.14 0.433 3.50 ± 0.86
 p-value (480 vs 30) 0.528 0.814 0.509
p27-pT198 30 0.25 ± 0.14 0.31 ± 0.15 0.929 0.852 0.30 ± 0.13
480 0.11 ± 0.06 0.22 ± 0.20 0.864 0.19 ± 0.16
 p-value (480 vs 30) 0.854 0.863 0.805
p38α-pT180/Y182 30 1.41 ± 0.09 2.36 ± 0.63 0.123 0.015 2.17 ± 0.69
480 1.29 ± 0.08 2.58 ± 1.27 0.057 2.15 ± 1.17
 p-value (480 vs 30) 0.882 0.644 0.909
p53-pS15 30 0.36 ± 0.06 0.91 ± 0.41 0.371 0.310 0.80 ± 0.44
480 0.29 ± 0.05 0.66 ± 0.58 0.576 0.54 ± 0.46
 p-value (480 vs 30) 0.926 0.606 0.728
p53-pS392 30 0.26 ± 0.12 0.53 ± 0.20 0.666 0.650 0.47 ± 0.21
480 0.13 ± 0.06 0.28 ± 0.34 0.826 0.23 ± 0.25
 p-value (480 vs 30) 0.865 0.601 0.676
p53-pS46 30 0.77 ± 0.17 1.24 ± 0.37 0.449 0.395 1.14 ± 0.38
480 0.56 ± 0.12 0.87 ± 0.50 0.644 0.77 ± 0.39
 p-value (480 vs 30) 0.788 0.446 0.531
p70S6K-pT389 30 0.36 ± 0.27 0.47 ± 0.25 0.853 0.708 0.45 ± 0.22
480 0.11 ± 0.08 0.33 ± 0.33 0.736 0.26 ± 0.27
 p-value (480 vs 30) 0.747 0.773 0.670
p70S6K-pT421/S424 30 0.72 ± 0.22 1.15 ± 0.43 0.483 0.294 1.06 ± 0.42
480 0.46 ± 0.14 0.99 ± 0.72 0.434 0.81 ± 0.59
 p-value (480 vs 30) 0.738 0.731 0.643
PDGF-Rβ-pY751 30 0.82 ± 0.18 1.31 ± 0.32 0.426 0.542 1.21 ± 0.35
480 1.11 ± 0.24 1.17 ± 0.65 0.921 1.15 ± 0.46
 p-value (480 vs 30) 0.707 0.783 0.859
PLC-γ1-pY783 30 0.46 ± 0.12 0.95 ± 0.43 0.431 0.370 0.85 ± 0.43
480 0.31 ± 0.08 0.65 ± 0.53 0.620 0.54 ± 0.42
 p-value (480 vs 30) 0.849 0.532 0.625
PRAS40-pT246 30 1.58 ± 0.41 2.81 ± 0.76 0.046 0.264 2.57 ± 0.86
480 2.30 ± 0.60 2.08 ± 0.61 0.751 2.15 ± 0.45
 p-value (480 vs 30) 0.357 0.127 0.989
PYK2-pY402 30 0.35 ± 0.13 0.75 ± 0.27 0.515 0.423 0.67 ± 0.29
480 0.20 ± 0.08 0.53 ± 0.43 0.624 0.42 ± 0.36
 p-value (480 vs 30) 0.851 0.650 0.691
RSK1/2/3-pS380/S386/S377 30 0.94 ± 0.42 0.92 ± 0.31 0.965 0.771 0.92 ± 0.27
480 0.49 ± 0.22 0.78 ± 0.34 0.664 0.69 ± 0.29
 p-value (480 vs 30) 0.562 0.781 0.522
SRC-pY419 30 2.75 ± 0.26 3.69 ± 0.45 0.127 0.043 3.50 ± 0.57
480 2.40 ± 0.23 3.32 ± 0.36 0.174 3.01 ± 0.59
 p-value (480 vs 30) 0.656 0.436 0.431
STAT2-pY689 30 3.08 ± 0.73 3.86 ± 0.71 0.074 0.796 3.71 ± 0.71
480 4.67 ± 1.10 3.72 ± 0.98 0.048 4.03 ± 0.90
 p-value (480 vs 30) 0.004 0.663 0.027
STAT3-pS727 30 0.19 ± 0.16 0.40 ± 0.19 0.735 0.698 0.36 ± 0.19
480 0.05 ± 0.04 0.20 ± 0.30 0.828 0.15 ± 0.23
 p-value (480 vs 30) 0.855 0.669 0.705
STAT3-pY705 30 0.32 ± 0.11 0.71 ± 0.31 0.522 0.429 0.63 ± 0.32
480 0.19 ± 0.07 0.52 ± 0.53 0.626 0.41 ± 0.42
 p-value (480 vs 30) 0.872 0.689 0.729
STAT5A-pY694 30 1.86 ± 0.27 3.26 ± 0.79 0.025 0.022 2.98 ± 0.92
480 2.30 ± 0.34 3.00 ± 0.94 0.295 2.77 ± 0.78
 p-value (480 vs 30) 0.579 0.595 0.845
STAT5A/B-pY694/Y699 30 0.88 ± 0.27 1.77 ± 0.42 0.150 0.209 1.59 ± 0.54
480 1.37 ± 0.42 1.63 ± 0.31 0.700 1.55 ± 0.26
 p-value (480 vs 30) 0.529 0.770 0.701
STAT5B-pY699 30 1.33 ± 0.29 2.76 ± 0.75 0.022 0.017 2.47 ± 0.91
480 1.81 ± 0.39 2.59 ± 1.35 0.249 2.33 ± 1.06
 p-value (480 vs 30) 0.542 0.723 0.737
STAT6-pY641 30 1.78 ± 0.50 2.94 ± 0.63 0.062 0.305 2.71 ± 0.75
480 2.67 ± 0.75 2.46 ± 1.20 0.749 2.53 ± 0.86
 p-value (480 vs 30) 0.254 0.314 0.654
TOR-pS2448 30 2.75 ± 0.20 3.13 ± 0.05 0.538 0.339 3.05 ± 0.17
480 2.48 ± 0.18 2.97 ± 0.62 0.463 2.81 ± 0.52
 p-value (480 vs 30) 0.732 0.750 0.646
WNK1-pT60 30 0.75 ± 0.07 0.56 ± 0.16 0.758 0.622 0.60 ± 0.16
480 0.85 ± 0.08 0.59 ± 0.02 0.699 0.68 ± 0.15
 p-value (480 vs 30) 0.896 0.948 0.884
YES-pY426 30 1.94 ± 0.19 2.8 ± 0.25 0.166 0.343 2.62 ± 0.44
480 2.22 ± 0.22 2.23 ± 0.49 0.986 2.23 ± 0.35
 p-value (480 vs 30) 0.713 0.24 0.766
β-Catenin 30 1.03 ± 0.06 1.28 ± 0.25 0.687 0.531 1.23 ± 0.24
480 0.95 ± 0.06 1.27 ± 0.41 0.631 1.16 ± 0.34
 p-value (480 vs 30) 0.912 0.983 0.916

Mean ± SD for the reference related values of assessed 45 phospho-proteins (as related to the signals of the reference spots × 100), and the respective p-values for the post/hoc effects of time and ACE I-allele (i.e., genotype). Bold values indicate effects which were deemed significant at p < 0.05. Phospho-proteins in bold demonstrated genotype effects. ANOVA with post/hoc test of least significant difference.