Fig 6. Loquacious interacts directly with DENV 3’UTR.

A. Electrophoretic mobility assay of Ae. aegypti Loqs-PA (left panel), Loqs-PB (right panel) and GFP control with a 117 bp ³²P labeled dsRNA corresponding to the firefly luciferase sequence. dsRNA was incubated with 5-fold dilutions of recombinant maltose-binding protein (MBP)-Loqs or MBP-GFP and complexes were resolved on native polyacrylamide gels. B. Quantification of (A) with dissociation constants for the indicated protein. C. Electrophoretic mobility assay of Ae. aegypti Loqs-PA (left panels) and Loqs-PB (right panels) with the indicated ssRNAs corresponding to DENV2 5’ UTR, NS1, NS5 and 3’ UTR sequences. Top panel indicates a schematic representation of the position of the probes on the DENV genome (not to scale). ssRNA was incubated with 2-fold dilutions of recombinant MBP-Loqs and complexes were resolved on native polyacrylamide gels. The images of the free ssRNA and the Loqs-RNA complex for the 5’ UTR are cropped from the same gel. Full images are provided in S5 Fig. D. Quantification of (C) with dissociation constants for the indicated RNAs.