Table 1.

Mutations and corresponding affinities and kinetic profiles evaluated in the SAR study

Position[a]	Compound[b]	KD	ka	kd
		[nM]	[106 M−1s−1]	[10−3 s−1]
	Cp40	0.8 ± 0.2	1.0 ± 0.4	0.7 ± 0.1
	Cp40[c]	0.5 ± 0.1	2.8 ± 0.5	1.4 ± 0.1
	Cp20	3.4 ± 0.6	1.3 ± 0.3	4.3 ± 1.3
1	Cp40 y1Y	2.5 ± 0.9	0.6 ± 0.3	1.3 ± 0.1
	Cp40 y1A	3.8 ± 0.5	0.4 ± 0.0	1.5 ± 0.4
	Cp40 y1a	2.9 ± 0.6	0.8 ± 0.1	2.3 ± 0.4
5	Cp40 (1MeW)5 W	19.2 ± 2.0	0.2 ± 0.0	2.7 ± 0.7
9	Cp40 Sar9G	1.7 ± 0.7	0.9 ± 0.4	1.2 ± 0.2
7	Cp40 D7E	2.4 ± 0.5	0.9 ± 0.2	1.9 ± 0.1
	Cp40 D7S	5.6 ± 1.3	0.1 ± 0.0	0.5 ± 0.2
12	Cp40 R12Cit	3.2 ± 0.6	0.6 ± 0.1	1.9 ± 0.0
	Cp40 R12K	1.6 ± 0.2	0.5 ± 0.1	0.8 ± 0.0
	Cp40 R12S	9.9 ± 0.5	0.2 ± 0.0	1.8 ± 0.3
7/12	Cp40 D7S/R12S	59.1 ± 41.3	0.1 ± 0.0	4.0 ± 1.0
14	Cp40 mI14T	6.2 ± 0.8	0.2 ± 0.0	1.4 ± 0.1
	Cp40 mI14A	1.2 ± 0.6	3.0 ± 1.6	3.0 ± 0.4
	Cp40 ΔmI14	1.8 ± 0.2	1.5 ± 0.2	2.7 ± 0.2

^aResidue position based on the Cp40 numbering, ^babbreviations used: 1MeW 1-methyl tryptophan, Sar sarcosine, ^creported values from Qu et al.¹⁴. Values represent average±standard deviation from three independent SPR experiments on amine-reactive immobilized C3b.