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. 2022 Sep 6;13:945409. doi: 10.3389/fimmu.2022.945409

Figure 2.

Figure 2

Decline in pre-DC number and altered pre-DC commitment in the BM during sepsis. WT mice underwent sham or CLP surgery and BM cells were isolated at indicated time points thereafter. (A) Gating strategy of pre-DCs (B220−CD11b−CD11c+MHCII−CD135+). Pre-DC subsets were distinguished according to the expression of Ly6C and Siglec-H: double-positive pre-DCs (dP pre-DCs; Ly6c+Siglec-H+), pDC-primed pre-DCs (Ly6c-Siglec-H+), pre-DC1 (Ly6c-Siglec-H−), and pre-DC2 (Ly6c+Siglec-H−). Representative dot plots of one sham and one CLP mouse (4 days after surgery) are shown. Numbers indicate the percentage in the respective quadrant. (B) Absolute number of pre-DCs per mouse BM. (C) Distribution of pre-DCs on dP pre-DCs, pDC-primed pre-DCs, pre-DC1, and pre-DC2. Data show the median with interquartile range (B) or median (C) of n = 4 (0 h; equivalent to naïve mice) or n = 7–14 mice per group. Significant differences were tested using Mann–Whitney U-test. *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 between sham and CLP mice. #p ≤ 0.05 versus “0 h”. pDC, plasmacytoid DC.