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. 2022 Sep 6;13:945409. doi: 10.3389/fimmu.2022.945409

Figure 6.

Figure 6

CD8+ T cells in the BM instruct differentiating DCs for cytokine secretion during sepsis. WT mice (n = 3–4 mice per group) were treated with anti-CD8β (αCD8β) or with the isotype control (iso) antibodies before induction of sepsis. Four days after CLP, BM cells were isolated and pooled to generate BM-derived DCs (BMDC). (A) Gating strategy for the analysis of CD40 and CD86 expression on CD11c+MHC II+ BMDC. (B) Cumulative data of CD40 and CD86 expression of three independent experiments. (C) BMDCs were stimulated with LPS or CpG and the release of IL-12p70, IL-10, and TNFα into the supernatant was quantified. Data show the mean ± SD of triplicate cultures of one representative experiment. Unstimulated BMDC did not secrete these cytokines (not shown). Statistically significant differences were tested using unpaired Student t-test. (D) Cumulative data of the ratio of IL-12/IL-10 secretion of three independent experiments. Statistically significant differences were tested using paired Student t-test. *p ≤ 0.05; **p ≤ 0.01.