Figure 9.

TLR2^−/− CD8⁺ T cells shape the function of differentiating DCs in the BM during sepsis. Sepsis was induced in WT mice after adoptive transfer of WT or TLR2^−/− CD8⁺ T cells as described in Figure 8 . After 4 days, BM cells were isolated and BM-derived DCs (BMDCs) were generated in vitro. (A) Release of IL-12p70, IL-10, and TNFα after stimulation of BMDC with LPS or CpG. Data show mean ± SD of triplicate cultures from one representative experiment. Unpaired t-test was performed for statistical analysis. (B) Ratio of IL-12/IL-10 secretion of BMDC from three independent experiments. As a reminder, the color intensity in the triangle illustrates the increased IFN-γ signaling in the BM after transfer of TLR2^−/− CD8⁺ T cells. *p ≤ 0.05; **p ≤ 0.01, ***p ≤ 0.001.