TABLE 1.
Sequence of Primers and Real-Time qPCR Running Program
| Gene | Primer | Sequence of Primers | Real-Time qPCR Running Program |
|---|---|---|---|
| IL-1β | Forward | TGCCACCTTTTGACAGTGATG | (1) Preincubation ➢ 95°C for 5 min (2) Amplication (50 cycles) ➢ 95°C for 10 s ➢ 56°C for 20 s ➢ 72°C for 20 s (3) Melting ➢ 95°C for 10 s ➢ 65°C for 60 s ➢ 97°C for 1 s (4) Cooling 37°C for 30 s |
| Reverse | AAGGTCCACGGGAAAGACAC | ||
| IL-6 | Forward | TAGTCCTTCCTACCCCAATTTCC | |
| Reverse | TTGGTCCTTAGCCACTCCTTC | ||
| TNF-α | Forward | CATCTTCTAAAATTCGAGTGACAA | |
| Reverse | TGGGAGTAGACAAGGTACAACCC | ||
| β-actin | Forward | AGTGTGACGTTGACATCCGT | |
| Reverse | GCAGCTCAGTAACAGTCCGC |