Table 2.

Summary statistical data for the qRT-PCR validation study.

Brain Region	Gene	Mean	SEM	P value (two tailed)
	Adcy1	−1.295	0.008	0.0007
	Arc	−1.448	0.022	0.0024
PFC	Homer1	−1.074	0.009	0.0141
	Mapk1	−1.067	0.004	0.0041
	Nr4a1	−1.775	0.065	0.0070
	Adcy1	−1.541	0.023	0.0018
	Arc	−1.237	0.010	0.0018
PVN	Homer1	−1.143	0.009	0.0043
	Mapk1	−1.057	0.007	0.0129
	Nr4a1	−2.125	0.020	0.0003
	Adcy1	−1.499	0.038	0.0057
	Arc	−1.956	0.040	0.0017
CeA	Homer1	−1.437	0.017	0.0016
	Mapk1	−1.107	0.005	0.0025
	Nr4a1	−2.296	0.092	0.0051
	Adcy1	−1.125	0.020	0.0253
	Arc	−1.357	0.029	0.0065
BLA	Homer1	1.030	0.012	0.1217
	Mapk1	1.147	0.012	0.0066
	Nr4a1	−1.281	0.064	0.0484
	Adcy1	−1.481	0.019	0.0016
	Arc	−1.370	0.031	0.0069
DH	Homer1	−1.192	0.019	0.0100
	Mapk1	−1.127	0.003	0.0004
	NAa1	−1.332	0.014	0.0018
	Adcy1	1.523	0.028	0.0029
	Arc	−1.220	0.028	0.0162
VH	Homer1	−1.038	0.024	0.2484
	Mapk1	−1.081	0.072	0.3772
	Nr4a1	−1.409	0.026	0.0040

Summary mean fold change (in relation to Sham rats; theoretical mean = +/−1) in mRNA levels for Adcy1, Arc, Homer1, Mapk1 and Nr4a1 genes in HF rats in the PFC, PVN, CeA, BLA, DH and VH.The mean, SEM and p values corresponding to each of the five genes validated in each of the six brain regions are provided. Brains from five rats were pooled per treatment condition and three independent replicates were performed.