| Antibodies |
| Human IgG1 isotype control |
BioXcell |
Cat#BE0297 |
| αTNFα |
BioXcell |
Cat#SIM0006 |
| αIL-4 |
BioXcell |
Cat#BE0240 |
| αIL-9 |
BioXcell |
Cat#BE0327 |
| αIL-5 |
BioXcell |
Cat#BE0198 |
| αIFN-γ |
BioXcell |
Cat#BE0235 |
| αTGF-β |
BioXcell |
Cat#BE0057 |
| αIL-3 |
R&D Systems |
Cat#MAB603 |
| αIL-13 |
R&D Systems |
Cat#MAB213 |
| αIL-17 |
R&D Systems |
Cat#AF-317-NA |
| αGM-CSF |
R&D Systems |
Cat#MAB215 |
| αIL-2 |
R&D Systems |
Cat#MAB202 |
| PE anti-DYKDDDDK Tag Antibody |
BioLegend |
Cat#637309 |
| Biotin anti-human Mesothelin |
BioLegend |
Cat#530203 |
| Bacterial and virus strains |
| Myxoma virus (MYXV) |
MYXV production by Dr. Grant McFadden’s lab |
N/A |
| vMyx-tdTomato (MYXV-tdTomato) |
MYXV-tdTomato production by Dr. Grant McFadden’s lab |
N/A |
| vMyx-Fluc (MYXV-Luc+) |
MYXV- Luc+ production by Dr. Grant McFadden’s lab |
N/A |
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| Biological samples |
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| Chemicals, peptides, and recombinant proteins |
| BYK204165 (PARP-1 inhibitor) |
MilliporeSigma |
Cat#B3188 |
| Y-27632 (ROCK inhibitor) |
MilliporeSigma |
Cat#Y0503 |
| lactoferrin (NETs inhibitor) |
MilliporeSigma |
Cat#L9507 |
| CA-074Me (CTSB inhibitor) |
MilliporeSigma |
Cat#205530 |
| PIK-III (VPS34 inhibitor) |
VWR |
Cat#103546-876 |
| Tat-Beclin1 D11 |
Novus Biologicals |
Cat#NBP2-49888 |
| siRNAs targeting the human Na+, K+-ATPase α1 subunit |
Invitrogen |
Cat#4390824 |
| Nontargeting siRNA |
Thermo Scientific |
Cat#D-001210-02-20 |
| MHC class I–restricted TRP1 (TAPDNLGYA) |
GenScript |
N/A |
| MHC class II-restricted TRP1 (SGHNCGTCRPGWRGAACNQKILTVR) |
GenScript |
N/A |
| Human IFN-γ |
R&D Systems |
Cat#285-IF |
| Human IL-2 |
R&D Systems |
Cat#202-IL |
| Critical commercial assays |
| Mouse IFN-gamma ELISpot Kit |
R&D Systems |
EL485 |
| Caspase-8 Activity Assay Kit (Colorimetric) |
Novus Biologicals |
Cat#NBP2-54817 |
| Human CD3+ T Cell Isolation Kit |
STEMCELL |
Cat#17751 |
| Human CD4+ T Cell Isolation Kit |
STEMCELL |
Cat#17952 |
| Human CD8+ T Cell Isolation Kit |
STEMCELL |
Cat#17953 |
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| Deposited data |
| RNA-Sequencing |
NCBI Gene Expression Omnibus |
GSE199777 |
| RNA-Sequencing |
NCBI Gene Expression Omnibus |
GSE200305 |
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| Experimental models: Cell lines |
| SK-OV-3 |
ATCC |
Cat#HTB-77 |
| Raji |
ATCC |
Cat#CCL-86 |
| PANC-1 |
ATCC |
Cat#CRL-1469 |
| SK-BR-3 |
ATCC |
Cat#HTB-30 |
| RPMI 8226 |
ATCC |
Cat#CRM-CCL-155 |
| B16 |
ATCC |
Cat#CRL-6323 |
| ID-8 |
A gift from Dr. Neveen Said at Wake Forest School of Medicine |
N/A |
| Mel-264 |
A gift from Dr. Steven Rosenberg |
N/A |
| U251 |
A gift from Dr. Waldemar Debinski. |
N/A |
| HUVECs |
ATCC |
Cat#PCS-100-013 |
| RWPE-1 |
ATCC |
Cat#CRL-11609 |
| HS-5 |
ATCC |
Cat#CRL-11882 |
| BSC40 |
ATCC |
Cat#CRL-2761 |
| 293T |
ATCC |
Cat#CRL-3216 |
| Experimental models: Organisms/strains |
| C57BL/6 |
The Jackson Laboratory |
Cat#000664 |
| Ifnar1−/− (B6(Cg)-Ifnar1tm1.2Ees/J) |
The Jackson Laboratory |
Cat#028288 |
| DKO-NSG (MHC-I/II-double knockout; NOD.Cg-B2mtm1Unc Prkdcscid H2dlAb1-Ea Il2rgtm1Wjl/SzJ) |
The Jackson Laboratory |
Cat#030547 |
| Cd4−/− (B6.129S2-Cd4tm1Mak/J) |
The Jackson Laboratory |
Cat#002663 |
| Cd8−/− (B6.129S2-Cd8atm1Mak/J) |
The Jackson Laboratory |
Cat#002665 |
| Cd3−/− (B6;129-Cd3etm1Lov/J) |
The Jackson Laboratory |
Cat#004177 |
| CD4+ TRP-1 (B6.Cg-Rag1tm1Mom Tyrp1B-w Tg(Tcra,Tcrb)9Rest/J) |
The Jackson Laboratory |
Cat#008684 |
| CD8+ TRP-1 (B6.Cg-Rag2tm1.1Cgn TcrbLn4Sdou TcraLn2Sdou/J) |
The Jackson Laboratory |
Cat#030957 |
| Oligonucleotides |
| mGAPDH F: 5’-TTGATGGCAACAATCTCCAC-3’ |
Sigma |
N/A |
| mGAPDH R: 5’-CGTCCCGTAGACAAAATGGT-3’ |
Sigma |
N/A |
| mTyrp1 F: 5’-CCCCTAGCCTATATCTCCCTTTT-3’ |
Sigma |
N/A |
| mTyrp1 R: 5’-TACCATCGTGGGGATAATGGC-3’ |
Sigma |
N/A |
| mCd3e F: 5’-TCAGCCTCCTAGCTGTTGG-3’ |
Sigma |
N/A |
| mCd3e R: 5’-GTCAACTCTACACTGGTTCCTG-3’ |
Sigma |
N/A |
| mCd8a F: 5’-CCGTTGACCCGCTTTCTGT-3’ |
Sigma |
N/A |
| mCd8a R: 5’-TTCGGCGTCCATTTTCTTTGG-3’ |
Sigma |
N/A |
| mCd4 F: 5’-CTAGCTGTCACTCAAGGGAAGA-3’ |
Sigma |
N/A |
| mCd4 R: 5’-CGAAGGCGAACCTCCTCTAA-3’ |
Sigma |
N/A |
| mCxcl10 F: 5’-CCAAGTGCTGCCGTCATTTTC-3’ |
Sigma |
N/A |
| mCxcl10 R: 5’-GGCTCGCAGGGATGATTTCAA-3’ |
Sigma |
N/A |
| mCcr7 F: 5’-CAGGTGTGCTTCTGCCAAGAT-3’ |
Sigma |
N/A |
| mCcr7 R: 5’-GGTAGGTATCCGTCATGGTCT-3’ |
Sigma |
N/A |
| mIl12a F: 5’-CAATCACGCTACCTCCTCTTTT-3’ |
Sigma |
N/A |
| mIl12a R: 5’-CAGCAGTGCAGGAATAATGTTTC-3’ |
Sigma |
N/A |
| mIl12b F: 5’-GTCCTCAGAAGCTAACCATCTCC-3’ |
Sigma |
N/A |
| mIl12b R: 5’-CCAGAGCCTATGACTCCATGTC-3’ |
Sigma |
N/A |
| mGzmb F: 5’-TCTCGACCCTACATGGCCTTA-3’ |
Sigma |
N/A |
| mGzmb R: 5’-TCCTGTTCTTTGATGTTGTGGG-3’ |
Sigma |
N/A |
| mSlc11a1 F: 5’-GCAGGCCCAGTTATGGCTC-3’ |
Sigma |
N/A |
| mSlc11a1 R: 5’-CAGGCTGAATGTACCCTGGTC-3’ |
Sigma |
N/A |
| mIfng F: 5’-GCCACGGCACAGTCATTGA-3’ |
Sigma |
N/A |
| mIfng R: 5’-TGCTGATGGCCTGATTGTCTT-3’ |
Sigma |
N/A |
| mLef1 F: 5’-GCCACCGATGAGATGATCCC-3’ |
Sigma |
N/A |
| mLef1 R: 5’-TTGATGTCGGCTAAGTCGCC-3’ |
Sigma |
N/A |
| hMSLN F: 5’-CCAACCCACCTAACATTTCCAG-3’ |
Sigma |
N/A |
| hMSLN R: 5’-CAGCAGGTCCAATGGGAGG-3’ |
Sigma |
N/A |
| hAtp1a1 F: 5’-ACAGACTTGAGCCGGGGATTA-3’ |
Sigma |
N/A |
| hAtp1a1 R: 5’-TCCATTCAGGAGTAGTGGGAG-3’ |
Sigma |
N/A |
| hSell F: 5’-ACCCAGAGGGACTTATGGAAC-3’ |
Sigma |
N/A |
| hSell R: 5’-GCAGAATCTTCTAGCCCTTTGC-3’ |
Sigma |
N/A |
| hIl7r F: 5’-CCCTCGTGGAGGTAAAGTGC-3’ |
Sigma |
N/A |
| hIl7r R: 5’-CCTTCCCGATAGACGACACTC-3’ |
Sigma |
N/A |
| hTcf7 F: 5’-CTGGCTTCTACTCCCTGACCT-3’ |
Sigma |
N/A |
| hTcf7 R: 5’-ACCAGAACCTAGCATCAAGGA-3’ |
Sigma |
N/A |
| hGzmb F: 5’-CCCTGGGAAAACACTCACACA-3’ |
Sigma |
N/A |
| hGzmb R: 5’-GCACAACTCAATGGTACTGTCG-3’ |
Sigma |
N/A |
| hPrf1 F: 5’-GGCTGGACGTGACTCCTAAG-3’ |
Sigma |
N/A |
| hPrf1 R: 5’-CTGGGTGGAGGCGTTGAAG-3’ |
Sigma |
N/A |
| hLag3 F: 5’-GCGGGGACTTCTCGCTATG-3’ |
Sigma |
N/A |
| hLag3 R: 5’-GGCTCTGAGAGATCCTGGGG-3’ |
Sigma |
N/A |
| hCtla4 F: 5’-GCCCTGCACTCTCCTGTTTTT-3’ |
Sigma |
N/A |
| hCtla4 R: 5’-GGTTGCCGCACAGACTTCA-3’ |
Sigma |
N/A |
| hPdcd1 F: 5’-CCAGGATGGTTCTTAGACTCCC-3’ |
Sigma |
N/A |
| hPdcd1 R: 5’-TTTAGCACGAAGCTCTCCGAT-3’ |
Sigma |
N/A |
| hGAPDH F: 5′ -AGTCAACGGATTTGGTCGTATTGGG-3′ |
Sigma |
N/A |
| hGAPDH R: 5′ -ACGTACTCAGCGCCAGCATCG-3′ |
Sigma |
N/A |
| Recombinant DNA |
| MART-127-35 specific TCR lentivector (DMF5) |
a gift from Dr. Steven Rosenberg |
N/A |
| PMKO.1 |
Addgene |
Cat#10676 |
| MigR1 |
Addgene |
Cat#27490 |
| MSCV-IRES |
Addgene |
Cat#20672 |
| pUltra |
Addgene |
Cat#24129 |
| lentiCRISPR v2 |
Addgene |
Cat#52961 |
| Software and algorithms |
| GSEA v2.2.2 |
Broad Institute |
http://software.broadinstitute.org/gsea/index.jsp
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| R |
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https://www.r-project.org/
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| Other |
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