FIG. 1.

Saturation curves for pyruvate and oxaloacetate for the wild-type and mutant L. pentosus l-LDHs. Reaction velocities for the wild-type (A) and P101N mutant (B) L. pentosus l-LDHs were measured in the presence of the indicated concentrations of pyruvate (open symbols) or oxaloacetate (closed symbols). Dashed and solid lines indicate the calculated saturation curves for pyruvate and oxaloacetate, respectively, by kinetic parameters shown in Table 1.