FIG. 2.

Saturation curves for pyruvate and oxaloacetate for L. casei l-LDH at pH 5.0. Reaction velocities were measured in the presence of the indicated concentrations of pyruvate (open symbols) or oxaloacetate (closed symbols), without (circles) or with (squares) 5 mM Fru(1,6)P₂. Dashed and solid lines indicate the calculated saturation curves for pyruvate and oxaloacetate, respectively, by kinetic parameters shown in Table 2.