TABLE 1.
Kinetic parameters for various substrates for L. pentosus l-LDH
| Substrate | Wild type
|
P101N
|
||||
|---|---|---|---|---|---|---|
| Km (mM) | Vmax (U/mg) | Vmax/Km (min−1 · mg−1) | Km (mM) | Vmax (U/mg) | Vmax/Km (min−1 · mg−1) | |
| Pyruvate | 1.8 | 1,250 | 690 | 4 | 940 | 235 |
| Oxaloacetate | 4.6 | 1,450 | 315 | 30 | 890 | 30 |
| Hydroxypyruvate | 4.1 | 1,450 | 350 | 20 | 840 | 42 |
| 2-Ketobutylate | 32 | 830 | 26 | 27 | 180 | 6.7 |
| Phenylpyruvate | 15 | 480 | 32 | 50 | 110 | 2.2 |
| 2-Ketovalerate | 23 | 75 | 3.3 | 75 | 22 | 0.29 |
| 2-Ketocaproate | 29 | 110 | 3.8 | 54 | 30 | 0.56 |
| 2-Ketoisocaproate | 44 | 4 | 0.1 | NDa | ND | ND |
| 2-Ketoglutarate | 20 | 8 | 0.4 | 100 | 4 | 0.04 |
a
ND, not determined (enzyme activity too weak for determination of exact values).