TABLE 2.
Kinetic parameters for pyruvate and oxaloacetate for L. casei l-LDH
| Condition | S0.5 (mM) | Vmax (U/mg) | Vmax/S0.5 (min−1 · mg−1) | h |
|---|---|---|---|---|
| pH 5.0 | ||||
| No Fru(1,6)P2 | ||||
| Pyruvate | 16.0 | 270 | 17 | 2.4 |
| Oxaloacetate | 18.0 | 120 | 7 | 3.1 |
| 5 mM Fru(1,6)P2 | ||||
| Pyruvate | 0.4 | 1,000 | 2,500 | 1 |
| Oxaloacetate | 1.1 | 625 | 570 | 1 |
| pH 7.0 | ||||
| 5 mM Fru(1,6)P2 | ||||
| Pyruvate | 30.0 | 560 | 19 | 2.4 |
| 5 mM Fru(1,6)P2 + 10 mM MnSO4 | ||||
| Pyruvate | 4.7 | 1,000 | 210 | 1 |
| Oxaloacetatea | 3.4 | 825 | 240 | 1 |
a
Kinetic parameters were calculated using the data for <10 mM oxaloacetate, since oxaloacetate was insoluble above 20 mM under these conditions.