Table 1. Fixed parameters in the model.

Parameter	Description	Value	Reference
nsyts	Number of syts per SV	15	Takamori et al., 2006
Mslots	Number of binding slots for syts to PI(4,5)P2 (see Figure 2)	Varied from 1 to 6	This paper
nves	Number of RRP vesicles	Mean: 4000, sd: 2000, gamma distribution	Wölfel and Schneggenburger, 2003
[Ca2+]0	Resting [Ca2+]i	0.05 µM	Helmchen et al., 1997
KD,2Ca2+	Dissociation constant of C2B for two Ca2+ ions	2212 µM2	van den Bogaart et al., 2012
KD,PIP2	Dissociation constant of C2B for PI(4,5)P2	20 µM	van den Bogaart et al., 2012
A	Allosteric factor see Materials and methods for calculation	0.00022	van den Bogaart et al., 2012
β	Ca2+ unbinding rate constant	KD,2Ca2+ ∙ α	Computed using best fit α (see Table 2)
δ	PI(4,5)P2 unbinding rate constant of C2B	KD,PIP2 ∙ γ	Computed using best fit α (see Table 2)
L+	Basal fusion rate	4.23 ∙10–4 s–1	Computed using data from Kochubey and Schneggenburger, 2011