FIG. 5.

Agarose gel electrophoresis of RT-PCR products amplified by primers from ADP1 grown on benzyl acetate and benzyl alcohol. The positions of the primers for spanning the areAB intergenic region (BAr, BAf) and the areCB intragenic region (CBr, CBf) are shown relative to the gene organization of areCBA. The values for molecular size markers (in base pairs) in lanes S (HyperLadder I; Bioline, London, United Kingdom) are indicated on the right side of the gel. Lanes: 1, areCB, benzyl acetate-grown cells (expected size, 953 bp); 2, areCB, benzyl acetate-grown cells digested with SacI (640 and 313 bp); 3, areCB, benzyl alcohol-grown cells (expected size, 953 bp); 4, areCB, benzyl alcohol-grown cells digested with SacI (640 and 313 bp); 5, areBA, benzyl acetate-grown cells (expected size, 946 bp); 6, areBA, benzyl acetate-grown cells digested with AccI (631 and 315 bp); 7, areBA, benzyl alcohol-grown cells (expected size, 946 bp); 8, areBA, benzyl alcohol-grown cells digested with AccI (631 and 315 bp). No detectable products were obtained in control reactions with each pair of primers, from which RT had been omitted, or in reactions carried out on succinate-grown cells (data not shown).