TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype and/or phenotype | Reference or source |
|---|---|---|
| Acinetobacter strains | ||
| ADP1 (BD413) | Wild type | 11 |
| ADPW56 | areC::Km; transformation of ADP1 with pADPW22 | 9 |
| ADPW61 | areA::lacZ-Km; transformation of ADP1 with pADPW38 | This study |
| ADPW63 | areC::lacZ-Km; transformation of ADP1 with pADPW47 | This study |
| ADPW79 | areR::Km; transformation of ADP1 with pADPW90 | This study |
| ACN274 | rpoN::Km derivative of ADP1 | E. L. Neidle |
| E. coli strains | ||
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17(rK− mK+) phoA supE44 λ−thi-1 gyrA96 relA1 | Gibco BRL |
| XL1-Blue MRF' | Δ(mcrA)183 Δ(mcrCB-hsdSMR-mrr)173′ endA1′ supE44′ thi-1′ recA1′ gyrA96′ relA1′ lac [F′ proAB lacIqZΔM15 Tn10 (Tetr)] | Stratagene |
| Plasmids | ||
| pUC18 | Apr, cloning vector | 28 |
| pUC18NP | pUC18 without PstI in the MCS | 27 |
| pUC4K | Apr Kmr; source plasmid for Kmr cassette | 26 |
| pKOK6.1 | Apr KmrlacZ; source plasmid for promoterless lacZ-Km cassette | 12 |
| pADPW32 | 8.0-kbp SacI fragment cloned from ADPW57 containing Kmr cassette and the whole of areA in pUC18 | This study |
| pADPW36 | 1.2-kbp EcoRIa fragment containing areA in pUC18 | This study |
| pADPW38 | pADPW36 with lacZ-Km cassette from pKOK6.1 cloned into NsiI site in areA | This study |
| pADPW45 | 6.2-kbp HindIII fragment cloned from ADPW56 containing areR and areC::Km in pUC18 | This study |
| pADPW46 | 6.2-kbp HindIII fragment cloned from ADPW56 containing areR and areC::Km in pUC18NP | This study |
| pADPW47 | pADPW46 with lacZ-Km cassette from pKOK6.1 cloned into PstI of the Kmr cassette | This study |
| pADPW71 | 3.5-kbp HindIII fragment from pADPW45 in pUC18 | This study |
| pADPW90 | pADPW71 with Kmr cassette from pUC4K cloned into the BglII site in areR | This study |
a
∗, restriction site added by PCR.