TABLE 2.
Specific activities of the Are enzymes in extracts of ADP1 and its derivatives
| Strain and growth substrate | Sp
acta (U/mg of protein) against benzyl substrates
|
||
|---|---|---|---|
| Benzaldehyde dehydrogenase | Benzyl alcohol dehydrogenase | Benzyl esterase | |
| ADP1 | |||
| Succinate | <0.04 | <0.04 | <0.04 |
| Benzaldehyde | 0.5 | 0.6 | 11 |
| Benzyl alcohol | 0.4 | 0.9 | 9 |
| Benzyl acetate | 0.26 | 0.7 | 8 |
| Succinate + benzyl alcohol | 0.24 | 0.33 | 6 |
| ADPW79 | |||
| Succinate + benzyl alcohol | <0.04 | <0.04 | 0.14 |
| ACN274 | |||
| Succinate + benzyl alcohol | <0.04 | 0.15 | 0.6 |
a
Specific activities were determined from three determinations at two protein concentrations on each of two cell extracts prepared from independent cultures.