Fig. 2.

Chronic methamphetamine administration affects the period of locomotor activity in UV LD conditions.

(A–D). Chronic MAP induced locomotor activity rhythm in UV LD conditions. Day 0 indicates the start day of MAP administration (0.005%). Each periodogram corresponds to a∼f in actograms. MAP was withdrawn at the time of triangle arrow in f (MAP-containing water bottle was replaced with the bottle containing pure water). Per1 expressions in the SCN were examined at * (ZT5) using LUC antibody in Fig. 2A. The period of locomotor activity rhythms are shown in a∼f. Per1 gene expression rhythms from ①∼⑩ in actogram in Fig. 2C are shown in Supplementary Figure S2. (E).Per1 expression in the SCN at ZT5 (* in Fig. 2A). Third ventricle (3V); optic chasm (OC); suprachiasmatic nucleus (SCN). (F). Day-night difference of Per1 expression in the SCN. Data are mean ± SDM (n = 3). Statistical significance as detected by student's t-test (***P < 0.001). The scale bar is 200 μm.