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. 2022 Feb 24;37(11):2643–2656. doi: 10.1007/s00467-022-05440-5

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Electron microscopy of NUP93 mutants and wild type Drosophila nephrocytes. Top panel shows low power sections, bottom panel shows higher power sections. NUP93-1: A collapsed cellular profile with indented margins and large vacuoles abundant in the cytoplasm. Basement membrane is intact with very few fenestrations leading into large interconnecting sub-membranous spaces containing membrane bound inclusions. NUP93-2: An over-inflated cellular profile with large vacuoles abundant in the cytoplasm. Basement membrane is intact with fewer fenestrations than control leading into a well-defined narrow sub-membranous labyrinth. Wild type control: An inflated cellular profile with large vacuoles abundant in the cytoplasm. Basement membrane is intact with abundant fenestrations leading into well-defined narrow sub-membranous labyrinths