TABLE 2.
Effects of the pharmacological NM‐II inhibitor, blebbistatin, on AJ and TJ structure and remodeling in vitro
| Cell line | Experimental conditions | Effects on epithelial junctions | References |
|---|---|---|---|
| Eph4 mammary epithelial cells | Confluent cell monolayers | No changes in TEER and fluorescein flux; no changes in the TJ structure | 97 |
| MDCK kidney epithelial cells | Confluent cell monolayers | Increased TEER; no changes in FITC dextran flux and TJ structure | 98 |
| T84 colonic epithelial cells | Confluent cell monolayers | Slightly decreased TEER | 20 |
| NRK kidney epithelial cells | Steady‐state monolayers | Disassembled punctate AJs and TJs | 99 |
| MCF‐7 mammary epithelial cells | Steady‐state monolayers | Disrupted steady‐state AJs | 100 |
| T84 cells | Calcium switch | Attenuated TJ reassembly; no effect on AJ reassembly | 18 |
| MDCK cells | Calcium switch | Prevented TJ reassembly | 101 |
| T84 cells | Calcium depletion | Attenuated AJ and TJ disassembly | 20 |
| T84 cells | Interferon‐γ treatment | Attenuated TJ disassembly | 102 |
| HPAF‐II pancreatic epithelial cells | Octylindolactam‐V treatment | Attenuated AJ and TJ disassembly | 103 |
| Kidney tubular epithelial cells | Angiotensin II treatment | Attenuated AJ disassembly | 104 |