FIGURE 3.

Synthetic maturation of induced pluripotent stem cell‐derived cardiomyocytes (iPSC‐CMs) with dynamically clamped I_K1 ensures consistent action potentials (APs) under 1‐Hz pacing conditions. (a) Spontaneous behaviour from a subpopulation of six cells (each with a different coloured trace) that indicates the level of heterogeneity and inconsistent presence of APs in the cell population. (b) I_K1 dynamic clamp applied to the same cells makes them appear more mature when paced at 1 Hz. (c and d) Histograms for the I_K1 dynamically clamped and paced AP resting membrane potential (−74.2 ± 2.8 mV) and action potential duration at 90% repolarization (142.0 ± 48.3 ms) for the 40 cells in this study