TABLE 1.
The optimized VC protocol correctly identifies strong drug blocks
| Drug | IKr | ICaL | INa | Ito | IK1 | If | IKs |
|---|---|---|---|---|---|---|---|
| Cisapride (125 nM) | 95%* | 1% | 2% | 13% | 5% | ?? | 2% |
| Verapamil (150 nM) | 21% | 39%* | <1% | 1% | 3% | ?? | 3% |
| Quinidine (2529 nM) | 89%* | 16% | 10% | 43%* | 1% | ?? | 27% |
| Quinine (12,000 nM) | 72%* | 29% | 28% | 15% | <1% | 32%* † | 20% |
Note: All percent block data, except quinine block of If, is taken from Crumb et al. (2016). The quinine block of If is from the HEK‐HCN1 experiments in this study. The optimized voltage clamp (VC) protocol qualitatively identifies drug blocks that Crumb et al. showed were greater than 30% and identifies funny current as a target of quinine (†).
*
P < 0.05, significantly greater than 30% block.