TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Genotype or relevant features | Reference or source |
|---|---|---|
| Strains | ||
| S. enterica serovar Typhimurium | ||
| LB5010 | Wild type; fimbriate with complete fim cluster | 8 |
| LBW100 | LB5010 fimW::kan Kanr | This study |
| ISF145 | LB5010 λfimA::lacZ lysogen | 58 |
| ISF145W | LBW100 λfimA::lacZ lysogen | This study |
| SL1344 | Wild type; fimbriate with complete fim cluster | 30 |
| SL1344JTW | SL1344 fimW::kan Kanr | This study |
| E. coli | ||
| SY327 | Host for suicide vector pGP704 | 46 |
| JM109λ145 | JM109 λfimA::lacZ lysogen | 66 |
| ER2508 | lon mutant for maltose-binding protein fusion expression | 39 |
| SU202 | JL1434λsulAlacZ lysogen | 15 |
| Plasmids | ||
| pISF101 | fimAICDHFZYWU, pACYC184 | 59 |
| pISF145 | fimA::lacZ reporter fusion, pMC1403 | 58 |
| pISF180 | fimZYW, pACYC184 | This study |
| pISF230 | fimZYW, pACYC184 | This study |
| pISF232 | fimW, pACYC184 | This study |
| pISF242 | malE::fimW fusion for FimW purification, pMalc2 | This study |
| pISF245 | lexA::fimZ fusion for two-hybrid system, pDP804 | This study |
| pISF248 | lexA::fimW fusion for two-hybrid system, pMS604 | This study |
| pISF252 | fimW::lacZ reporter fusion, pMC1403 | This study |
| pISF253 | fimW::lacZ reporter fusion single copy, pGS375 | This study |