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Expression of β-galactosidase by λfimA-lacZ reporters in serovar Typhimurium and E. coli
| Strain | Plasmid (relevant genotype) | β-Galactosidase expression (Miller units)a |
|---|---|---|
| S. enterica serovar Typhimurium | ||
| IS145 | None | 30 |
| IS145W | None | 74 |
| E. coli | ||
| JM109λfimA-lacZ | None | 0 |
| JM109λfimA-lacZ | pISF180 (fimZ+Y+W+) | 570 |
| JM109λfimA-lacZ | pISF230 (fimZ+Y+W) | 1,400 |
β-Galactosidase activity is reported in Miller units (45), and all assays were performed at least three times with ≤20% variability.
