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Protein secondary structures containing EVs derived from Raphanus sativus L. var. caudatus Alef microgreens were determined by (a) curve-fitting with the 50% Gaussian and Lorentzian function in the amide I and amide II regions (RMS error 0.002). (b) The relative amount of protein secondary structure was determined by specific area integration. Different letters indicate a statistical difference between groups (p-value < 0.05).
