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. 2022 Sep 20;13:5513. doi: 10.1038/s41467-022-33173-4

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a A BRET assay to examine the constitutive activity of GPR110 CTF. Data are presented as mean values ± SD; n = 3 independent samples. **P < 0.01; ***P < 0.001. Data between WT and mutants were analyzed by two-sided test (for G_q group, P < 0.001; for G_s group, P < 0.001; for G_i group, P = 0.0012; for G₁₃ group, P < 0.001). Source data are provided as a Source Data file. b The stalk peptide (100 μM) of GPR110 increases neurite outgrowth. c Measurement of neurite outgrowth. n = 3, 20 cells were analyzed per well, *P <0.05. All data were analyzed by two-sided, one-way ANOVA with Tukey’s test (from left to right, P = 0.0233, P = 0.0194). Source data are provided as a Source Data file.