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. 2022 Sep 20;13(9):806. doi: 10.1038/s41419-022-05243-4

Fig. 5. Validation of upregulated signal transducer and activator of transcription 1 (STAT1) and death receptor 5 (DR5) signaling following kinesin family member 3A (KIF3A) knockdown in the AT/RT cell lines BT‐12 and CHLA‐266.

Fig. 5

a, b siRNA‐mediated knockdown of KIF3A in BT‐12 (a) and CHLA‐266 (b) cells was achieved using siPOOLs. mRNA expression was normalized to the housekeeping genes glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) and phosphoglycerate kinase 1 (PGK1) and calculated relative to siCTRL. Values shown represent mean ± SEM of three biologically independent replicates. *p < 0.05; **p < 0.01; ***p < 0.001 (t‐test). c Representative Western blot images for KIF3A, pSTAT1 (Tyr701), STAT1, DR5 and actin as a loading control following siRNA‐mediated KIF3A knockdown. d, e Gene expression data are derived from the Tumor ATRT ‐ Kool ‐ 49 ‐ MAS5.0 ‐ u133p2 data set available at the R2: Genomics analysis and visualization platform (http://r2.amc.nl).