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. 2022 Sep 20;17:92. doi: 10.1186/s11671-022-03729-w

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a–b Confocal fluorescence images (a) and flow cytometry data (b) of SMMC-7721 cells after incubation with free Ce6, MCP, and MCPF for 4 h. c The cytotoxicity of MCPF on SMMC-7721 cells without laser irradiation. d Cell viability of SMMC-7721 cells treated with free Ce6 or MCPF under single or combined laser irradiation (660 nm at 500 mW cm⁻² and 808 nm at 1.5 W cm⁻²). e The ROS-producing capability of MCPF NPs detected by flow cytometry using DCFH-DA as an indicator