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. 2022 Sep 7;13:961909. doi: 10.3389/fphys.2022.961909

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Copyright © 2022 Li, Wang, Ying, Zheng, Tan, Yu, Gong, Li, Deng, Yang, Li and Jiang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Stattic inhibited STAT3 and enhanced the anti-inflammatory and anti-apoptosis effects in EA pre-treatment sepsis mice. (A,B) Serum concentrations of TNF-α and IL-1β. (C,D) mRNA expression of Tnf-α and Il-1β. (E) Representative images of western blots forBAX,Bcl2, IL-6, TNF-α, IL-1β, and GAPDH. (F) BAX/Bcl2 ratio. (G–I) Quantitative results of IL-6, TNF-α, and IL-1β. (J–K) TUNEL staining and Quantitative analysis, apoptosis positive cells (red), nuclei (blue). N = 4 per group. EA, electroacupuncture; LPS, lipopolysaccharide. *p < 0.05 vs. control group, ^# p < 0.05 vs. LPS group,^† p < 0.05 vs. LPS + EA group.