Figure 1.

Expression systems for recombinant GF production

(A) Small-scale protein expression screening used to identify the expression vector and host strain combination capable of facilitating cytoplasmic soluble protein expression. The band corresponding to the protein of interest is marked with (∗). T - total cell lysate; S - soluble fraction. Molecular weight markers are labeled to the right of each gel image.

(B) Expression vector and host strain combinations for successful expression and purification of soluble, bioactive growth factors. (ˆ) denotes instances where the use of SHuffle T7 Express was required for soluble expression of some orthologs.