FIG. 2.

Effect of cell growth rate and carbon substrate on total fumarase activity in batch culture. (A) W3110 cells were grown in minimal medium with the indicated carbon compounds (40 mM) or in buffered LB. Solid bars, FumA+B activities; open bars, FumC activities. (B) W3110 cells were grown in the indicated medium, and the cell generation time as well as the total fumarase activities were recorded. Medium and carbon compound (40 mM) used for cell growth: 1, LB plus glucose; 2, LB; 3, glucose plus Casamino Acids; 4, glucose; 5, glycerol; 6, xylose; 7, fumarate; 8, succinate; 9, galactose; 10, acetate. Units of fumarase activity are expressed as micromoles of fumarate formed per minute at 25°C and pH 7.3.