TABLE 1.
Mediuma | Fumarase activityb (U/mg) | Generation timec (min) |
---|---|---|
LB + glucose | 0.02 | 21 |
LB + pyruvate | 0.02 | 23 |
LB | 0.03 | 23 |
Glucose + Casamino Acids | 0.03 | 40 |
Glucose | 0.03 | 47 |
Xylose | 0.03 | 71 |
Glycerol | 0.03 | 84 |
Acetate | — | NG |
Succinate | — | NG |
Fumarate | — | NG |
Cells were grown aerobically in a minimal basal medium (pH 7.0) with the indicated carbon compounds added (40 mM).
Units are expressed as micromoles of fumarate formed per minute at 25°C and pH 7.3.
NG, no growth.