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. 2022 Jul 19;14(5):983–1001. doi: 10.1016/j.jcmgh.2022.07.010

Figure 1.

Figure 1

Leptin suppressed gluconeogenesis in hepatocytes. (A) Sites for primers in Obr cDNA. F1 and R1, forward and reverse primers for Obr; F2 and R2, forward and reverse primers for Obrb; F3, forward primer for Obra and Obrc; R3, reverse primer for Obra; R4, reverse primer for Obrc. (B) The absolute mRNA levels of Obra, Obrb, and Obrc in mouse tissues and primary hepatocytes. Phosphorylation level of (C) STAT3, (D) glucose production, and (E) gene expression levels of Pepck1, G6pc, and Pgc1a in 80 ng/mL leptin or vehicle-treated primary mouse hepatocytes. Phosphorylation level of (F) STAT3, (G) glucose production, and (H) gene expression levels of Pepck1, G6pc, and Pgc1a in 80 ng/mL leptin or vehicle-treated Hepa 1–6 cells. N = 3 for each group of pot graphs. ∗P < .05, ∗∗P < .01 compared with the control group. Results represent 1 of 3 independently performed experiments. pSTAT3, phospho-STAT3.